《植物生理学报》 2013, 49(12): 1421-1426

锁阳愈伤组织诱导和增殖及不定根分化

岳鑫, 段园园, 陈贵林*

内蒙古大学生命科学学院, 内蒙古自治区中蒙药材规范化生产工程技术研究中心, 呼和浩特010021

通信作者：陈贵林；E-mail: guilinchen@aliyun.com；Tel: 0471-4992577

摘　要：

以药用寄生植物锁阳的不同部位肉质茎为外植体, 研究外植体形态及植物生长调节剂配比对愈伤组织形成、增殖及不定根分化的影响, 建立了高效的锁阳肉质茎愈伤组织诱导、增殖和不定根分化体系。结果表明, 锁阳茎下部大小为1.5 cm×1.5 cm×1.5 cm的外植体, 维管束平行于培养基放置, 有利于愈伤组织形成; 外植体培养50 d, 愈伤组织形成。高效的愈伤组织诱导培养基为MS+6-BA 1.0 mg·L^-1+2,4-D 3.0 mg·L^-1, 愈伤组织诱导率可达67%; 增殖培养基为MS+6-BA 0.5 mg·L^-1+2,4-D 1.5 mg·L^-1, 增殖系数为74%; 在MS+6-BA 1.0 mg·L^-1+NAA 2.0 mg·L^-1分化培养基中, 不定根诱导率达56%。

关键词：药用寄生植物; 肉质茎; 不定根; 愈伤组织

收稿：2013-09-05   修定：2013-10-21

资助：国家科技支撑计划课题(2011BAI07B07)、高等学校博士点基金(20091501110002)、国家自然科学基金(30660015)和内蒙古大学“211工程”创新人才培养计划

In vitro Callus Induction, Proliferation and Adventitious Root Organogenesis of Cynomorium songaricum

YUE Xin, DUAN Yuan-Yuan, CHEN Gui-Lin*

The Good Agriculture Practice Engineering Technology Research Center of Chinese and Mongolian Medicine in Inner Mongolia, College of Life Sciences, Inner Mongolia University, Hohhot 010021, China

Corresponding author: CHEN Gui-Lin; E-mail: guilinchen@aliyun.com; Tel: 0471-4992577

Abstract:

Effects of different explants and plant growth regulators on callus formation, proliferation and differentiation were studied to establish high-performance tissue culture method of succulent stems of medicinalparasitic plant Cynomorium songaricum. The results indicated that the lower part of the succulent stems were of highest callus formation rate with the size 1.5 cm×1.5 cm×1.5 cm and vascular bundle parallel placing. The optimal callus induction medium was MS+1.0 mg·L^-1 6-BA+3.0 mg·L^-1 2,4-D, the inductivity of which would be 67%. It was MS medium containing 0.5 mg·L^-1 6-BA and 1.5 mg·L^-1 2,4-D that promoted the proliferation of callus with the proliferation coefficient 74%. It was MS medium including 1.0 mg·L^-1 6-BA and 2.0 mg·L^-1 NAA that benefited the differentiation of callus to adventitious root with the differentiation ratio 56%.

Key words: medicinal-parasitic plant; succulent stems; adventitious root; callus